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Cryo-EM structure of the transposon-associated TnpB enzyme

By: Ryoya Nakagawa ยทย Hisato Hirano ยทย Satoshi N. Omura ยทย Suchita Nety ยทย Soumya Kannan ยทย Han Altae-Tran ยทย Xiao Yao ยทย Yuriko Sakaguchi ยทย Takayuki Ohira ยทย Wen Y. Wu ยทย Hiroshi Nakayama ยทย Yutaro Shuto ยทย Tatsuki Tanaka ยทย Fumiya K. Sano ยทย Tsukasa Kusakizako ยทย Yoshiaki Kise ยทย Yuzuru

Nature, Published online: 05 April 2023; doi:10.1038/s41586-023-05933-9

Cryo-electron microscopy analysis of the Deinococcus radiodurans ISDra2 TnpB in complex with its cognate ฯ‰RNA and target DNA provides insights into the mechanism of TnpB function and the evolution of CRISPRโ€“Cas12 effectors.
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