Large-scale mapping and mutagenesis of human transcriptional effector domains

Nature, Published online: 05 April 2023; doi:10.1038/s41586-023-05906-y

A high throughput recruitment assay testing the transcriptional activity of more than 100,000 protein fragments tiling across most human chromatin regulators and transcription factors maps the locations and strengths of activation, repression and bifunctional domains, and identifies the sequences necessary for these functions.